A HUMAN MONOCLONAL-ANTIBODY WHICH INHIBITS THE COAGGREGATION ACTIVITYOF PORPHYROMONAS-GINGIVALIS

Authors
ABIKO Y OGURA N MATSUDA U YANAGI K TAKIGUCHI H
Citation
Y. Abiko et al., A HUMAN MONOCLONAL-ANTIBODY WHICH INHIBITS THE COAGGREGATION ACTIVITYOF PORPHYROMONAS-GINGIVALIS, Infection and immunity, 65(9), 1997, pp. 3966-3969
Citations number
28
Categorie Soggetti
Immunology,"Infectious Diseases
Journal title
Infection and immunity → ACNP
ISSN journal
00199567
Volume
65
Issue
9
Year of publication
1997
Pages
3966 - 3969
Database
ISI
SICI code
0019-9567(1997)65:9<3966:AHMWIT>2.0.ZU;2-O
Abstract
A B-cell line producing a human monoclonal antibody (HuMAb) against a recombinant 40-kDa outer membrane protein (OMP) of Porphyromonas gingi valis was constructed by in vivo immunization of a severe combined imm unodeficiency C.B.-17/Icr mouse, which had been injected with human pe ripheral blood lymphocytes, with recombinant 40-kDa OMP and subsequent Epstein-Barr virus immortalization of B cells isolated from the splee n of the mouse. This HuMAb inhibited coaggregation between P. gingival is vesicles and Actinomyces naeslundii cells.