IMMUNOENZYME ASSESSMENT OF HUMAN APO-B-LIPOPROTEIN BINDING TO IMMOBILIZED RECEPTOR OF LOW-DENSITY LIPOPROTEINS .1. PREPARATION OF ANTIRECEPTOR MONOCLONAL-ANTIBODIES

The preparation and properties of V5 monoclonal antibody to low densit y lipoprotein receptor (LDL-receptor) from bovine adrenal cortex membr anes are described, The monospecific V5 antibody recognizes the LDL-re ceptor (the only protein with molecular mass of 140 kD) in bovine adre nal cortex membranes. V5 antibody fails to compete with human low dens ity lipoproteins (LDL) for binding to the LDL-receptor. After absorpti on in standard 96-well polystyrene plates, V5 antibody efficiently bin ds the affinity-purified LDL-receptor from the solution and the subseq uent binding of the LDL-receptor with human LDL was determined using p eroxidase-labelled antibodies to apolipoprotein B. The LDL-receptor im mobilized by V5 antibodies is suggested for use in studies on the bind ing of human lipoproteins to the LDL-receptor.