Gg. Borisenko et al., PHOTOCHEMICAL-REACTIONS OF NITROSYL HEMOGLOBIN DURING EXPOSURE TO LOW-POWER LASER IRRADIATION, Biochemistry, 62(6), 1997, pp. 661-666
Relaxation of blood vessel walls is one of the physiological effects i
nduced by visible radiation. This effect is similar to the endothelium
-derived relaxation (based on endothelium-derived relaxing factor, EDR
F) and depends on the presence of nitric oxide donors, We assumed that
nitric oxide hemoglobin (HbNO) might participate in the mechanism of
this phenomenon and investigated the photochemical reactions using low
-power He-Cd (441.6 nm) and He-Ne (632.8 nm) lasers. It was found that
in the presence of oxygen the He-Cd laser but not He-Ne laser induced
a photolytic decrease of the HbNO EPR signal amplitude by 25-35%. Usi
ng optical absorption measurements, we showed that methemoglobin was f
ormed during irradiation. The comparison of the kinetics of this proce
ss and the irreversible reduction of paramagnetic centers suggest that
the laser radiation leads to dissociation of the heme-NO complexes, b
ut not to oxidation of the heme iron. In the absence of oxygen the pho
todissociation effect did not exceed 5-10% and was reversible. We supp
ose that this finding can be explained by fast reassociation of NO wit
h unligated Hb. The EPR spectrum of iminonitroxide was observed upon i
rradiation of HbNO with nitronyl nitroxide in a vacuum, suggesting the
formation of free NO. Thus, our experiments confirm that NO-hemoglobi
n can reversibly dissociate and release free NO during exposure to He-
Cd laser radiation.