LINKAGE OF TATA-BINDING PROTEIN AND PROTEASOME SUBUNIT C5 GENES IN MICE AND HUMANS REVEALS SYNTENY CONSERVED BETWEEN MAMMALS AND INVERTEBRATES

The TATA-binding protein (TBP) is a factor required for the transcript ion of all classes of eukaryotic genes. Here, we demonstrate that in t he mouse the TBP-encoding gene (Tbp) resides next to the proteasomal s ubunit C5-encoding gene (Psmb1). The genes are located on mouse chromo some 17 in the t complex within the Hybrid sterility 1 (Hst1) region. We demonstrate that the homologous human genes (TBP AND PSMB1) are tig htly linked on the long arm of chromosome 6, in a region syntenic with the proximal part of mouse chromosome 17. The mouse Tbp and Psmb1 and the human TBP and PSMB1 genes are transcribed in the opposite orienta tion. The TATA-binding protein and proteasomal subunit C5 genes are al so linked on chromosome III of Caenorhabditis elegans, and together th ey are linked to other genes whose homologs map to human chromosome 6 and mouse chromosome 17. In the Drosophila genome, the housekeeping TA TA-binding protein gene maps close to two other genes with homologs in the mammalian major histocompatibility complex. There thus exists con served synteny of unrelated genes between mammals and invertebrates. ( C) 1997 Academic Press.