CLONING, STRUCTURAL-ANALYSIS, AND CHROMOSOMAL LOCALIZATION OF THE HUMAN CSRP2 GENE ENCODING THE LIM DOMAIN PROTEIN CRP2

The CSRP2 gene encoding the LIM domain protein CRP2 was originally ide ntified in quail based on its strong transcriptional suppression in tr ansformed avian fibroblasts. Here we have isolated a human CSRP2 cDNA clone encoding a 193-amino-acid human CRP2 (hCRP2) protein with 96.4% amino acid sequence identity to the avian homolog. The CSRP2 cDNA clon e was used to isolate CSRP2-related clones from gamma EMBL3 and P1 lib raries of human genomic DNA. The complete organization of the CSRP2 ge ne was determined by nucleic acid hybridization, transcriptional mappi ng, and nucleotide sequence analysis. The gene spans a total of approx imately 22 kb and contains six exons. The coding region is confined to exons 2-6 and predicts a hCRP2 protein identical in its amino acid se quence to the protein deduced from the CSRP2 cDNA clone. By fluorescen ce in situ hybridization using both lambda EMBL3 and P1 library clones as hybridization probes and a new method for computerized signal loca lization, CSRP2 was mapped to chromosome subband 12q21.1, a region fre quently affected by deletion or breakage events in various tumor types . The library screens also led to the isolation of a CSRP2-related pse udogene, CSRP2P, which carried several extensive deletions and nucleot ide substitutions but no intervening sequences in comparison to the CS RP2 cDNA sequence. By physical linkage and fluorescence in situ hybrid ization, CSRP2P was mapped to chromosome subband 3q21.1. (C) 1997 Acad emic Press.