LOCALIZATION OF THE GENE CODING FOR MYOSIN PHOSPHATASE, TARGET SUBUNIT-1 (MYPT1) TO HUMAN-CHROMOSOME 12Q15-Q21

Myosin phosphatase target subunit 1 (MYPT1), which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits o f myosin phosphatase, Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho, as pr eviously shown (K. Kimura, et al., 1996, Science 273: 245-248). To und erstand the role of MYPT1 in the regulation of the cytoskeleton in hum an diseases, we have cloned a 4855-bp cDNA for human MYPT1 using the r at MYPT1 cDNA as probe. Sequencing analysis has revealed that human MY PT1 contains 1030 amino acid residues with a calculated molecular mass of approximately 115 kDa, Fluorescence in situ hybridization analysis placed the human MYPT1 gene on chromosome 12q15-q21.2. Radiation hybr id mapping has shown that the human MYPT1 gene is located very near th e highly polymorphic marker CHLC.GATA65A12, which lies between D12S350 and D12S106. (C) 1997 Academic Press.