N. Takahashi et al., LOCALIZATION OF THE GENE CODING FOR MYOSIN PHOSPHATASE, TARGET SUBUNIT-1 (MYPT1) TO HUMAN-CHROMOSOME 12Q15-Q21, Genomics, 44(1), 1997, pp. 150-152
Myosin phosphatase target subunit 1 (MYPT1), which is also called the
myosin-binding subunit of myosin phosphatase, is one of the subunits o
f myosin phosphatase, Myosin phosphatase regulates the interaction of
actin and myosin downstream of the guanosine triphosphatase Rho, as pr
eviously shown (K. Kimura, et al., 1996, Science 273: 245-248). To und
erstand the role of MYPT1 in the regulation of the cytoskeleton in hum
an diseases, we have cloned a 4855-bp cDNA for human MYPT1 using the r
at MYPT1 cDNA as probe. Sequencing analysis has revealed that human MY
PT1 contains 1030 amino acid residues with a calculated molecular mass
of approximately 115 kDa, Fluorescence in situ hybridization analysis
placed the human MYPT1 gene on chromosome 12q15-q21.2. Radiation hybr
id mapping has shown that the human MYPT1 gene is located very near th
e highly polymorphic marker CHLC.GATA65A12, which lies between D12S350
and D12S106. (C) 1997 Academic Press.