BENEFICIAL EFFECT OF MEDIUM WITH HIGH-CONCENTRATION SERUM FOR DIRECT SPERM INJECTION INTO MOUSE OOCYTES USING A CONVENTIONAL PIPETTE

Authors
SUZUKI K YANAGIMACHI R
Citation
K. Suzuki et R. Yanagimachi, BENEFICIAL EFFECT OF MEDIUM WITH HIGH-CONCENTRATION SERUM FOR DIRECT SPERM INJECTION INTO MOUSE OOCYTES USING A CONVENTIONAL PIPETTE, Zygote, 5(2), 1997, pp. 111-116
Citations number
19
Categorie Soggetti
Developmental Biology","Cell Biology
Journal title
Zygote → ACNP
ISSN journal
09671994
Volume
5
Issue
2
Year of publication
1997
Pages
111 - 116
Database
ISI
SICI code
0967-1994(1997)5:2<111:BEOMWH>2.0.ZU;2-X
Abstract
Although the piezo-electrically driven pipette is much more effective than a conventional pipette for direct sperm injection into mouse oocy tes, the piezo-pipette driving unit is expensive and may not be readil y accessible to most basic science laboratories. We report here that t he inefficiency of the conventional pipette can be improved by perform ing the operation in a medium with high serum concentration.