Ac. Ronse et al., EFFECT OF ENVIRONMENTAL-FACTORS ON IN-VITRO GROWTH AND MORPHOLOGY OF OTACANTHUS-COERULEUS (SCROPHULARIACEAE), Belgian journal of botany, 130(1), 1997, pp. 3-7
In order to determine the most important environmental conditions for
the micropropagation of the ornamental Otacanthus coeruleus, the effec
ts of some macro and micro environmental factors on in vitro growth an
d morphogenesis were analysed. The growth increased both with higher t
emperature (day/night 25/20 degrees C) and irradiance (55 mu mole.m(-2
).s(-1)), and it was found to be the highest in the medium containing
6 g.l(-1) agar, 30 g.l(-1) sucrose and 10.7 g.l(-1) mannitol, with a m
acronutrient concentration equal to that of Murashige and Skoog's medi
um. The morphology of the explants was not so influenced by the tested
temperature and irradiance levels, but by the agar and sucrose concen
tration, and especially by the macronutrient concentration.