ONLINE ELECTROGENERATED RU(BPY)(3)(3-BLOCKERS SEPARATED WITH CAPILLARY ELECTROPHORESIS() CHEMILUMINESCENT DETECTION OF BETA)

A novel chemiluminescence (CL) detection scheme for capillary electrop horesis (CE) has been developed based on the Ru(bpy)(3)(3+) CL reactio n. Ru(bpy)(3)(2+) is incorporated into the CE electrolyte and an elect rode inserted into the outlet end of the capillary electrochemically o xidizes the Ru(bpy)(3)(2+) into the active Ru(bpy)(3)(3+) form, which then reacts with analytes at the end of the CE column and produces lig ht. The performance of the system is characterized for a series of bet a-blockers, with an LOD for oxprenolol of 0.6 mu g mL(-1) and a separa tion efficiency of 15000.