PROTEIN EXPRESSION IN RESPONSE TO FOLATE STRESS IN ESCHERICHIA-COLI

Interruption of folate metabolism by trimethoprim results in the eleva ted expression of folate stress proteins in Escherichia coli. E. coli grown in culture medium supplemented with the folate-dependent metabol ites glycine, methionine, and the purine nucleoside inosine shows redu ced expression of folate stress proteins, The folate stress proteins i nclude the universal Stress protein, the ferric uptake regulatory repr essor, and possibly, lipoamide dehydrogenase, the L protein component of the glycine cleavage enzyme complex.