ATP LEVELS AND GLUTATHIONE REGENERATION IN CANINE ERYTHROCYTES

The effect of ATP levels on GSH regeneration was examined in canine er ythrocytes. The main findings were: (1) The GSH regeneration was depen dent on glucose and ATP; (2) cytochalasin B and polymyxin B, both gluc ose transport inhibitors, reduced ATP synthesis and GSH regeneration; (3) inosine, a substrate of the salvage pathway, was not effective for ATP synthesis and GSH regeneration. These results indicate that gluco se transport and its metabolism play an important role in oxidant defe nce systems in general and GSH regeneration in particular in canine er ythrocytes.