EFFECTS OF SOME ANTINEOPLASTIC DRUGS (VINCRISTINE, DOXORUBICIN AND EPIRUBICIN) ON HUMAN PLATELET-AGGREGATION

The effects of some antineoplastic drugs (vincristine, doxorubicin and epirubicin) on collagen- and ADP-induced human platelet aggregation a re investigated. Platelet rich plasma (PRP) and platelet poor plasma ( PPP) from healthy male and female donors were used. The PRP was adjust ed with analogous PPP to 300,000 platelets/mul. Platelet aggregation w as studied according to Born's turbidimetric technique using an Aggrec order II PA 3220 with collagen at a concentration of 10 mug/ml and ADP at a concentration of 30 muM. Vincristine, doxorubicin and epirubicin significantly (p<0.01) inhibited collagen- and ADP-induced platelet a ggregation. The vincristine induced inhibition was higher than that in duced by doxorubicin or epirubicin. The effects of doxorubicin and epi rubicin were more intense on ADP-induced platelet aggregation than on the collagen induced one. Moreover, the doxorubicin inhibition of ADP- induced platelet aggregation was greater than the epirubicin one. In c onclusion, our study shows that vincristine, doxorubicin and epirubici n inhibit human platelet aggregation. The present results may improve the therapeutic use of these drugs since it has been clearly shown tha t drugs with antiplatelet activity could block metastases.