THE BINDING OF CORTICOSTERONE TO THE CLASS-THETA GLUTATHIONE-S-TRANSFERASE FROM THE EYES OF THE SHRIMP PENAEUS-JAPONICUS (CRUSTACEA, DECAPODA)

Authors
SHUFEN T CHUANG NN
Citation
T. Shufen et Nn. Chuang, THE BINDING OF CORTICOSTERONE TO THE CLASS-THETA GLUTATHIONE-S-TRANSFERASE FROM THE EYES OF THE SHRIMP PENAEUS-JAPONICUS (CRUSTACEA, DECAPODA), Comparative biochemistry and physiology. B. Comparative biochemistry, 108(2), 1994, pp. 215-219
Citations number
40
Categorie Soggetti
Biology
Journal title
Comparative biochemistry and physiology. B. Comparative biochemistry → ACNP
ISSN journal
03050491
Volume
108
Issue
2
Year of publication
1994
Pages
215 - 219
Database
ISI
SICI code
0305-0491(1994)108:2<215:TBOCTT>2.0.ZU;2-T
Abstract
The anionic homodimeric theta glutathione S-transferase (EC 2.5.1.18), with specific activities of 4.6 units/mg with 1-chloro-2,4-dinitroben zene as substrate, from the eyes of the shrimp Penaeus japonicus, bind s to corticosterone, which was confirmed by the analysis of the conjug ate by SDS-PAGE and fluorography of proteins with bound H-3-corticoste rone. The reaction of the theta glutathione S-transferase with cortico sterone was prevented by the presence of glutathione at 10 mM.