SMAD4 AND FAST-1 IN THE ASSEMBLY OF ACTIVIN-RESPONSIVE FACTOR

Members of the TGF-beta superfamily of signalling molecules work by ac tivating transmembrane receptors with phosphorylating activity (serine -threonine kinase receptors)(1); these in turn phosphorylate and activ ate(2) SMADs(3,4), a class of signal transducers. Activins are growth factors that act primarily through Smad2(5-7), possibly in partnership with Smad4, which forms heteromeric complexes with different ligand-s pecific SMADs after activation(8,9). In frog embryos, Smad2 participat es in an activin-responsive factor (ARF), which then binds to a promot er element of the Mix.2 gene(10). The principal DNA-binding component of ARF is FAST-1 (ref. 11), a transcription factor with a novel winged -helix structure. We now report that Smad4 is present in ARF, and that FAST-1, Smad4 and Smad2 co-immunoprecipitate in a ligand-regulated fa shion. We have mapped the site of interaction between FAST-1 and Smad2 /Smad4 to a novel carboxyterminal domain of FAST-1, and find that over expression of this domain specifically inhibits activin signalling. In a yeast two-hybrid assay,the FAST-1 carboxy terminus interacts with S mad2 but not Smad4. Deletion mutants of the FAST-1 carboxy terminus th at still participate in ligand-regulated Smad2 binding no longer assoc iated with Smad4 or ARF. These results indicate that Smad4 stabilizes a ligand-stimulated Smad2-FAST-1 complex as an active DNA-binding fact or.