A shallow hydrothermal brine seep located off the creek island of Mile
s in the Aegean Sea was studied. The brine fluid outcropped as a pool
of water in a seabed depression and was detected in the surrounding po
re-waters of sediments colonised by the sulphur bacterium Achromatium
volutans. The seep fluid was highly saline and sulphidic, depleted in
Mg2+ and SO42-, but enriched over seawater in Na+, Ca2+, K+, Cl-, SiO2
, reduced species and dissolved gases. The high concentrations of Na+,
Ca2+ and K+ were consistent with the Miles tectonic setting. Na-K and
Na-K-Ca geothermometers predicted a reservoir temperature of 300-325
degrees C for the most concentrated seep samples. The deep geothermal
reservoir within the metamorphic basement of Miles island has already
been located and studied and may represent the source of the seep flui
d. Faunal diversity was lowest in seep-influenced sediments, but a sul
phide-intolerant species was found in areas of the bacterial mat where
salinity and temperature were much lower. Pressure-induced variations
in the vertical depth of the brine interface may be occurring in the
sediment.