Pea aphids, Acyrthosiphon pisum, were fed on artificial diet containin
g various concentrations of melatonin. Under long-day conditions (16 h
light: 8 h dark) their progeny included males and virginoparous/ovipa
rous (asexual/sexual) intermediate females, which normally occur only
in short days or around critical night-length. Endogenous melatonin in
pea aphids was measured by radioimmunoassay and verified by paralleli
sm with a melatonin standard curve and by thin layer chromatography. H
owever, melatonin titres showed large variations and although they ten
ded to be higher during the scotophase than during the photophase they
were not significantly different. The possibility of melatonin being
involved in photoperiodism is discussed. (C) 1997 Elsevier Science Ltd
.