EFFECTS OF CYTOKINE-INDUCED NEUTROPHIL CHEMOATTRACTANTS (CINCS) ON SHAPE CHANGE, ADHESIVENESS AND PHAGOCYTOSIS OF RAT NEUTROPHILS

Rat cytokine-induced neutrophil chemoattractants (CINC)-1, -2 alpha, - 2 beta and -3 belong to alpha-chemokines and are known to be potent ch emoattractants for rat neutrophils in vivo and in vitro. In this study , the abilities of CINCs to affect several functions of rat neutrophil s are estimated, All CINCs at a concentration of 10(-8) M induced the polarization of rat neutrophils. The adhesion of each CINC-treated neu trophil to fibrinogen significantly increased, reaching a maximal adhe sion at 10(-8) M of each CINC. The CINCs enhanced the phagocytosis of heat-killed yeast cells by neutrophils in a dose-dependent manner, In contrast, CINCs did not induce the production of reactive nitrogen int ermediates, The results suggest that CINCs play a significant role in neutrophil infiltration and phagocytosis at inflammatory sites in rats .