A SINGLE-DOSE IMMUNIZATION WITH RABBIT HEMORRHAGIC-DISEASE VIRUS MAJOR CAPSID PROTEIN PRODUCED IN SACCHAROMYCES-CEREVISIAE INDUCES PROTECTION

Authors
BOGA JA ALONSO JMM CASAIS R PARRA F
Citation
Ja. Boga et al., A SINGLE-DOSE IMMUNIZATION WITH RABBIT HEMORRHAGIC-DISEASE VIRUS MAJOR CAPSID PROTEIN PRODUCED IN SACCHAROMYCES-CEREVISIAE INDUCES PROTECTION, Journal of General Virology, 78, 1997, pp. 2315-2318
Citations number
17
Categorie Soggetti
Virology,"Biothechnology & Applied Migrobiology
Journal title
Journal of General Virology → ACNP
ISSN journal
00221317
Volume
78
Year of publication
1997
Part
9
Pages
2315 - 2318
Database
ISI
SICI code
0022-1317(1997)78:<2315:ASIWRH>2.0.ZU;2-J
Abstract
The gene coding for the major capsid protein (VP60) from rabbit haemor rhagic disease virus was expressed in Saccharomyces cerevisiae under t he phosphoglycerate kinase promoter. The recombinant VP60 produced in yeast was antigenically similar to the viral polypeptide as determined with a polyclonal serum. Electron microscopic observation of the reco mbinant yeast-derived antigen revealed the presence of virus-like part icles similar in size and appearance to native capsids. Subcutaneous v accination of rabbits with a single dose of this antigen in the absenc e of commercial adjuvants conferred complete protection against the ha emorrhagic disease.