N. Inomata et al., MOLECULAR EVOLUTION OF THE AMY MULTIGENES IN THE SUBGENUS SOPHOPHORA OF DROSOPHILA, Molecular biology and evolution, 14(9), 1997, pp. 942-950
Molecular evolution of the Amy multigenes in Drosophila was investigat
ed using PCR amplification. Twenty-five partial Amy sequences from 13
species belonging mainly to the subgenus Sophophora were determined, a
nd a molecular phylogeny of the Amy genes in Drosophila was constructe
d, together with published Amy sequences. Clusterings of species are m
ostly consistent with the traditional classification and that inferred
from other genes. From sequence divergence between PCR products, seve
ral species, including D, elegans and D, fuyamai, were suggested to ha
ve multiple copies of the Amy genes. The loss of an intron took place
at least three times after the Sophophora radiation. In order to inves
tigate the mechanism of sequence evolution, the numbers of amino acid
replacement and synonymous substitutions in five lineages were estimat
ed. The heterogeneity in the relative numbers of synonymous and replac
ement substitutions among the lineages was found. Possible roles of se
lection in the sequence evolution of the Amy gene are discussed.