THE TRANS-SIALIDASE OF TRYPANOSOMA-CRUZI IS ANCHORED BY 2 DIFFERENT LIPIDS

Authors
AGUSTI R COUTO AS CAMPETELLA OE FRASCH ACC DELEDERKREMER RM
Citation
R. Agusti et al., THE TRANS-SIALIDASE OF TRYPANOSOMA-CRUZI IS ANCHORED BY 2 DIFFERENT LIPIDS, Glycobiology, 7(6), 1997, pp. 731-735
Citations number
40
Categorie Soggetti
Biology
Journal title
Glycobiology → ACNP
ISSN journal
09596658
Volume
7
Issue
6
Year of publication
1997
Pages
731 - 735
Database
ISI
SICI code
0959-6658(1997)7:6<731:TTOTIA>2.0.ZU;2-0
Abstract
The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [H-3]-palmitic acid and purified by im munoprecipitation with a monoclonal antibody. The action of PI-PLC on the immunoprecipitate released a lipid that was analyzed by TLC. Lyso- 1-O-hexadecylglycerol and N-palmitoyl-sphinganine were obtained in a 1 :3 ratio, A comparison with the GPI anchors present in the different s tages of T. cruzi was made.