BRADYKININ INDUCES ACTIN REORGANIZATION AND ENHANCES CELL MOTILITY INHACAT KERATINOCYTES

Citation
Kd. Coutant et al., BRADYKININ INDUCES ACTIN REORGANIZATION AND ENHANCES CELL MOTILITY INHACAT KERATINOCYTES, Biochemical and biophysical research communications, 237(2), 1997, pp. 257-261
Citations number
33
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006291X
Volume
237
Issue
2
Year of publication
1997
Pages
257 - 261
Database
ISI
SICI code
0006-291X(1997)237:2<257:BIARAE>2.0.ZU;2-4
Abstract
In HaCaT keratinocytes bradykinin-triggered actin reorganization was i nhibited by quinacrine, a phospholipase A(2) inhibitor, and restored b y addition of arachidonic acid, Bradykinin-induced actin breakdown and cortical actin formation were respectively prevented by indomethacin, a cyclooxygenase inhibitor, and nordihydroguaiaretic acid, a lipoxyge nase inhibitor. Addition of prostaglandins or leukotrienes, respective ly, reversed the effects of inhibitors. This suggested a crucial role for a cyclooxygenase product in actin depolymerization and for a lipox ygenase product in cortical actin formation. Furthermore, we found tha t bradykinin stimulated HaCaT keratinocyte migration. This event was b lock;ed by quinacrine, indomethacin or nordihydroguaiaretic acid, and restored by addition of prostaglandins or leukotrienes, respectively. We also showed that genistein, a tyrosine kinase inhibitor, inhibited HaCaT cell locomotion. In conclusion, bradykinin modulated actin reorg anization and cell motility in keratinocytes, probably by a mechanism involving arachidonic acid metabolites and a tyrosine kinase activity. (C) 1997 Academic Press.