EFFECT OF SILKWORM HEMOLYMPH ON THE EXPRESSION OF ESCHERICHIA-COLI BETA-GALACTOSIDASE IN INSECT-CELL LINES INFECTED WITH RECOMBINANT BACULOVIRUSES

The effects of silkworm hemolymph on the expression of foreign genes b y recombinant baculoviruses in cell lines were studied, The expression efficiency of beta-galactosidase by recombinant virus containing the E. coli lacZ gene at various concentrations of hemolymph and FBS was d etermined in BmN and Sf cell lines, The addition of hemolymph to the m edium containing FBS accelerated the expression of beta-galactosidase by recombinant viruses in both cells. It was more effective in BmN cel ls than in Sf cells. Hemolymph was most effective in enhancing virus m ultiplicity under conditions of 5% FBS.