Bap. Buscher et al., ONLINE ELECTRODIALYSIS CAPILLARY ZONE ELECTROPHORESIS MASS-SPECTROMETRY OF INOSITOL PHOSPHATES IN COMPLEX MATRICES, Journal of chromatography, 777(1), 1997, pp. 51-60
Citations number
30
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Electrodialysis has been coupled to capillary zone electrophoresis-mas
s spectrometry for on-line clean-up and analyte concentration. Two dif
ferent electrodialysis devices have been developed, each with its spec
ific features. The first device consisting of a donor and an acceptor
compartment, separated by a membrane with a cut-off of M-r 30 000, off
ers selectivity based on molecular mass, shape and charge. The second
device consists of three compartments, separated by membranes with a c
ut-off of M-r 500 and 30 000, respectively. In addition to selectivity
this device enables analyte enrichment between the two membranes. The
developed methods have been applied to the determination of inositol
phosphates in fermentation broth and in blood plasma. (C) 1997 Elsevie
r Science B.V.