PRIMED IN-SITU (PRINS) LABELING WITH ALU AND SATELLITE PRIMERS FOR RAPID CHARACTERIZATION OF HUMAN-CHROMOSOMES IN HYBRID CELL-LINES

The primed in situ (PRINS) labelling method was developed as an altern ative to classical cytogenetics and fluorescence in situ hybridization (FISH) for the characterization of interspecific somatic hybrids. Ful l karyotypes were performed by PRINS using Aluspecific primers to gene rate the painting of all human material associated with R-like banding . The representativity of individual human chromosomes was established using primers specific for discriminent alpha-satellite DNA sequences providing specific signals on the centromeres of the targeted chromos omes and corresponding spots in interphase nuclei. Using this methodol ogy, a somatic hybrid clone was shown to be monochromosomal for the de r(ll) from a t(11;22) patient.