I. Jagiello et al., NIPP-1, A NUCLEAR INHIBITORY SUBUNIT OF PROTEIN PHOSPHATASE-1, HAS RNA-BINDING PROPERTIES, The Journal of biological chemistry, 272(35), 1997, pp. 22067-22071
NIPP-1 is a nuclear inhibitory subunit of protein phosphatase-1 with s
tructural similarities to some proteins involved in RNA processing, We
report here that baculovirus-expressed recombinant NIPP-1 displays RN
A-binding properties, as revealed by North-Western analysis, by UV-med
iated cross-linking, by RNA mobility-shift assays, and by chromatograp
hy on poly(U)- Sepharose, NIPP-1 preferentially bound to U-rich sequen
ces, including RNA-destabilizing AUUUA motifs. NIPP-1 also associated
with single-stranded DNA, but had no affinity for double-stranded DNA.
The binding of NIPP-1 to RNA was blocked by antibodies directed again
st the COOH terminus of NIPP-1, but was not affected by prior phosphor
ylation of NIPP-1 with protein kinase A or casein kinase-2, which decr
eases the affinity of NIPP-1 for protein phosphatase-1. The catalytic
sub unit of protein phosphatase-1 did not bind to poly(U)Sepharose, bu
t it bound very tightly after complexation with NIPP-1. These data are
in agreement with a function of NIPP-1 in targeting protein phosphata
se-1 to RNA.