Ra. Smith et al., THE ROLE OF PUTATIVE FIBRINOGEN A-ALPHA-CHAIN, B-BETA-CHAIN, AND GAMMA-A-CHAIN INTEGRIN BINDING-SITES IN ENDOTHELIAL CELL-MEDIATED CLOT RETRACTION, The Journal of biological chemistry, 272(35), 1997, pp. 22080-22085
In this study, endothelial cell-mediated clot retraction was supported
by fibrin generated from several purified fractions of plasma fibrino
gen, purified proteolytic fragments of plasma fibrinogen, recombinant
normal fibrinogen, and recombinant variant fibrinogen, These results w
ere surprising because some of these fibrinogens lack domains that are
known binding sites for the integrin receptors that support clot retr
action. Specifically, fibrinogens lacking A alpha-chain RGD residues a
t 572-574 or lacking the gamma-chain residues AGDV 408-411 supported e
ndothelial cell-mediated clot retraction as well as intact fibrinogen,
Thus, clot retraction mediated by endothelial cells is not dependent
on either of these sites, A variety of monoclonal antibodies against t
he integrin alpha(v) beta(3) partially inhibited the endothelial cell-
mediated retraction of clots formed from plasma fibrinogen, As expecte
d, an antibody to the platelet integrin alpha(IIb)beta(3) did not inhi
bit endothelial cell-mediated clot retraction. These results indicate
that this retraction is mediated at least in part by alpha(v) beta(3).
These results support the conclusion that (a) neither of the two fibr
inogen cell binding sites described above is required to support clot
retraction or that (b) either site alone or in conjunction with other
fibrin(ogen) region(s) can support clot retraction, Thus, endothelial
cell-mediated clot retraction appears to be dependent on fibrinogen ce
ll binding sites other than those required to support adhesion of rest
ing platelets to immobilized fibrinogen and platelet aggregation.