R. Rouhana et al., ELECTROCHEMICAL STUDIES OF THE INTERFACIAL BEHAVIOR OF ALPHA-LACTALBUMIN AND BOVINE SERUM-ALBUMIN, Food research international, 30(1), 1997, pp. 13-20
Knowledge of the conformational behaviour of proteins as a function of
temperature is important in the thermal processing of foods. Equally
important is their interfacial behaviour with surfaces under these sam
e processing conditions. This paper describes electrochemical techniqu
es developed to study these structurally related properties of protein
s. The interfacial behaviour of alpha-lactalbumin and bovine serum alb
umin was studied on a platinum electrode using cyclic voltammetry over
a temperature range from 273 to 363 K. It was found that both protein
s adsorbed strongly on the metal surface. As the temperature was incre
ased up to the denaturation temperature of the protein, the surface ch
arge density for protein adsorption also increased due to the conforma
tional unfolding. Above the temperature for denaturation, the surface
charge density continued to increase. Mixtures of the two proteins res
ulted in a substantial increase in surface charge density above the de
naturation temperatures. A comparison of surface concentrations (Gamma
) of cyclic voltammetric measurements with those for ellipsometric mea
surements reported in the literature showed a similar behaviour with i
ncreasing adsorption with temperature. A value of 17 +/- 4 carboxylate
groups for the alpha-lactalbumin protein was determined from an analy
sis of the surface coverage which is consistent with the number of aci
dic residues on the protein. (C) 1997 Published by Elsevier Science Lt
d on behalf of the Canadian Institute of Food Science and Technology.