NADPH-DEPENDENT REDUCTION OF ETHYL ACETOACETATE COUPLED WITH ETHANOL OXIDATION IN KLOECKERA-MAGNA

Authors
KOMETANI T SAKAI Y URAI H YOSHII H MATSUNO R
Citation
T. Kometani et al., NADPH-DEPENDENT REDUCTION OF ETHYL ACETOACETATE COUPLED WITH ETHANOL OXIDATION IN KLOECKERA-MAGNA, Bioscience, biotechnology, and biochemistry, 61(8), 1997, pp. 1370-1372
Citations number
14
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
61
Issue
8
Year of publication
1997
Pages
1370 - 1372
Database
ISI
SICI code
0916-8451(1997)61:8<1370:NROEAC>2.0.ZU;2-S
Abstract
We evaluated the catalytic ability of 29 yeast strains to reduce ethyl acetoacetate (EA) in the presence of ethanol or glucose. In 18 yeast strains, the reduction in the presence of ethanol proceeded as well as in the presence of glucose, Among them, Kloeckera magna (AKU 4704) ef fectively catalyzed the NADPH-dependent reduction of EA in the presenc e of ethanol, In this reduction, 1 mol of EA was reduced by consuming 1 mol of ethanol, We found that the NADPH regeneration system responsi ble for EA reduction in K. magna was coupled with the oxidation of ace taldehyde to acetic acid catalyzed by an NADP(+)-dependent aldehyde de hydrogenase.