SUBSITE AFFINITIES OF ALPHA-GLUCOSIDASES FROM SPINACH SEEDS

The rate parameters for maltooligosaccharides of two alpha-glucosidase s from spinach seeds were examined and subsite affinities were evaluat ed, The subsite affinities for subsites 1, 2, 3, 4, 5, 6, and 7 in the active site of alpha-glucosidase A and B were 1.73, 2.91, 1.10, 0.25, 0.94, 0.03, and -0.01 kcal/mol, and 0.33, 4.94, 0.26, 0.14, -0.24, -0 .52, and 0.14 kcal/mol, respectively, The six and four subsites exist in alpha-glucosidase A and B, respectively, which maltooligosaccharide s or soluble starch could be bound.