UNIQUE INHIBITION OF MILTPAIN, A NEW CYSTEINE PROTEINASE FROM THE MILT OF CHUM SALMON, BY O-PHENANTHROLINE, PHENANTHRENEQUINONE, PHENAZINE,AND ACRIDINE

Authors
KAWABATA C ICHISHIMA E
Citation
C. Kawabata et E. Ichishima, UNIQUE INHIBITION OF MILTPAIN, A NEW CYSTEINE PROTEINASE FROM THE MILT OF CHUM SALMON, BY O-PHENANTHROLINE, PHENANTHRENEQUINONE, PHENAZINE,AND ACRIDINE, Bioscience, biotechnology, and biochemistry, 61(8), 1997, pp. 1405-1407
Citations number
12
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
61
Issue
8
Year of publication
1997
Pages
1405 - 1407
Database
ISI
SICI code
0916-8451(1997)61:8<1405:UIOMAN>2.0.ZU;2-6
Abstract
Miltpain (EC 3.4.22.-) is a new cysteine proteinase that has been isol ated from the milt of chum salmon (Oncorhynchus keta), and is strongly inhibited by cysteine proteinase inhibitors such as E-64, iodoacetami de, NEM, and PCMB. This paper deals with a unique feature, that the en zyme becomes inhibited by o-phenanthroline, phenanthrenequinone, phena nthrene, phenazine, and acridine which have some structural resemblanc e to a hydrophobic planar shape. Dixon plots showed that the inhibitio ns of the enzyme by o-phenanthroline and phenanthrenequinone were nonc ompetitive.