STRUCTURAL-ANALYSES OF XYLOGLUCAN HEPTASACCHARIDE BY THE POST-SOURCE DECAY FRAGMENT METHOD USING MATRIX-ASSISTED LASER DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY/
T. Yamagaki et al., STRUCTURAL-ANALYSES OF XYLOGLUCAN HEPTASACCHARIDE BY THE POST-SOURCE DECAY FRAGMENT METHOD USING MATRIX-ASSISTED LASER DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY/, Bioscience, biotechnology, and biochemistry, 61(8), 1997, pp. 1411-1414
In the post-source decay (PSD) fragment spectrum of a reduced xylogluc
an heptasaccharide (XXXGol) from tamarind seeds, eleven sodium-adduct
fragment ions and a precursor ion [M + Na](+) mere clearly observed by
using matrix-assisted laser desorption/ionization time-of-flight mass
spectrometry (MALDI-TOFMS). Each fragment ion interval corresponded t
o the absence of unhydroxylose, unhydroglucose, and glucitol residues,
indicating that PSD fragmentation cleavage in the sugar compound occu
rred only at glycosidic linkages close to the oxygen atom of saccharid
e ring members, and not in inner sugar ring bonds, The PSD fragment lo
lls sere classified into two series, one involving the reducing end an
d the other involving the non-reducing end, Structural information fro
m both the reducing and non-reducing ends could therefore br simultane
ously obtained from the measurement of the positive ion mode, Almost a
ll the fragment ions from species larger than trisaccharide residues c
ould be detected in this PSD fragment experiment, Such fragmentation i
nformation will enable the structural determination of xyloglucan olig
osaccharides.