A SIMPLE DNA EXTRACTION METHOD FOR PCR-BASED DETECTION OF XANTHOMONAS-CAMPESTRIS PV PELARGONII IN GERANIUMS

Citation
Ma. Sulzinski et al., A SIMPLE DNA EXTRACTION METHOD FOR PCR-BASED DETECTION OF XANTHOMONAS-CAMPESTRIS PV PELARGONII IN GERANIUMS, Journal of phytopathology, 145(5-6), 1997, pp. 213-215
Citations number
5
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
09311785
Volume
145
Issue
5-6
Year of publication
1997
Pages
213 - 215
Database
ISI
SICI code
0931-1785(1997)145:5-6<213:ASDEMF>2.0.ZU;2-3
Abstract
A simple method for PCR-based plant clinical diagnosis of bacterial bl ight of geraniums caused by Xanthomonas campestris pv. pelargonii is d escribed. The method entails maceration of infected tissues in water o r 10 mM Tris-HCl, pH 8.0 buffer, followed by treatment of the macerate with a commercially-available extraction matrix (GeneReleaser(TM)) in which nucleic acid is released by brief microwave heating. Nucleic ac id prepared in this manner served directly as template for PCR amplifi cation with primers targeting a sequence in the genome of the bacteriu m. Using this protocol, it was possible to quickly identify X. campest ris pv. pelargonii in infected geraniums, whereas amplification produc ts were not obtained with nucleic acid preparations from noninfected p lants, or from plants infected with the bacterial pathogens, Corynebac terium fascians or Pseudomonas cichorii.