KI-67 ANTIGEN RETRIEVAL IN FORMALIN-FIXED OR ETHANOL-FIXED, PARAFFIN-EMBEDDED TISSUES - AN ENHANCEMENT METHOD FOR IMMUNOHISTOCHEMICAL STAINING WITH AUTOCLAVE TREATMENT

Authors
TSUJI Y KUSUZAKI K HIRASAWA Y SERRA M BALDINI N
Citation
Y. Tsuji et al., KI-67 ANTIGEN RETRIEVAL IN FORMALIN-FIXED OR ETHANOL-FIXED, PARAFFIN-EMBEDDED TISSUES - AN ENHANCEMENT METHOD FOR IMMUNOHISTOCHEMICAL STAINING WITH AUTOCLAVE TREATMENT, Acta histochemica et cytochemica, 30(3), 1997, pp. 251-255
Citations number
8
Categorie Soggetti
Cell Biology
Journal title
Acta histochemica et cytochemica → ACNP
ISSN journal
00445991
Volume
30
Issue
3
Year of publication
1997
Pages
251 - 255
Database
ISI
SICI code
0044-5991(1997)30:3<251:KARIFO>2.0.ZU;2-O
Abstract
We developed a new immunohistochemical method to visualize Ki-67 antig en in formalin-fixed, paraffin-embedded sections of bone tumors. The m ethod uses autoclave pretreatment of sections immersed in citrate buff er before incubation with the MIB 1 antibody, which reacts with the Ki -67 gene product. MIB 1 antibody showed strong and discrete staining o f cell nuclei in proliferating tumor cells. This method also retrieved antigen in paraffin-embedded sections of alcohol-fixed tissue. Theref ore, tissues previously considered unsuitable for immunohistochemical analysis can be studied after autoclave treatment.