KI-67 ANTIGEN RETRIEVAL IN FORMALIN-FIXED OR ETHANOL-FIXED, PARAFFIN-EMBEDDED TISSUES - AN ENHANCEMENT METHOD FOR IMMUNOHISTOCHEMICAL STAINING WITH AUTOCLAVE TREATMENT
Y. Tsuji et al., KI-67 ANTIGEN RETRIEVAL IN FORMALIN-FIXED OR ETHANOL-FIXED, PARAFFIN-EMBEDDED TISSUES - AN ENHANCEMENT METHOD FOR IMMUNOHISTOCHEMICAL STAINING WITH AUTOCLAVE TREATMENT, Acta histochemica et cytochemica, 30(3), 1997, pp. 251-255
We developed a new immunohistochemical method to visualize Ki-67 antig
en in formalin-fixed, paraffin-embedded sections of bone tumors. The m
ethod uses autoclave pretreatment of sections immersed in citrate buff
er before incubation with the MIB 1 antibody, which reacts with the Ki
-67 gene product. MIB 1 antibody showed strong and discrete staining o
f cell nuclei in proliferating tumor cells. This method also retrieved
antigen in paraffin-embedded sections of alcohol-fixed tissue. Theref
ore, tissues previously considered unsuitable for immunohistochemical
analysis can be studied after autoclave treatment.