BIOTECHNOLOGICAL APPLICATIONS OF SURFACE-PLASMON RESONANCE

The surface plasmon resonance technique allows direct, real-time kinet ic measurements of the interaction of unlabelled biological molecules at surfaces. After a brief discussion of the principles of surface pla smon resonance, we review its application to the nonspecific adsorptio n of protein, the formation of phospholipid bilayers, membrane-protein interactions and DNA hybridization. The morphological studies of the biological surfaces using surface plasmon resonance microscopy and the potential of the surface plasmon resonance to measure the dynamics of surface layer heterogeneity are discussed.