A MODEL APPROACH TO DISTINGUISH DIPLOID AND TRIPLOID FISH BY MEANS OFCOMPUTER-ASSISTED IMAGE-ANALYSIS

A model approach to distinguish diploid and triploid fish rapidly upon computer-assisted image analysis of erythrocyte nuclear dimensions in blood smear samples was exemplified on measurements of 13 diploid and 13 artificially-induced triploid wels (Silurus glanis). Analysis of v ariance test confirmed that nuclear area, perimeter, nuclear major axi s and average nuclear size were the decisive dimensions for such appro ach (P < 0.0001). The respective values (mean +/- SD) for diploids and triploids were registered as follows: nuclear area 11.09 +/- 1.26 mu m(2) and 17.34 +/- 2.09 mu m(2); nuclear perimeter 12.67 +/- 0.80 mu m and 16.24 +/- 1.07 mu m; nuclear major axis 4.62 +/- 0.35 mu m and 6. 17 +/- 0.47 mu m and average nuclear size 4.01 +/- 0.22 mu m and 5.12 +/- 0.31 mu m. This approach was found a rapid and precise alternative to other currently used methods of ploidy level identification in fis h.