During central nervous system (CNS) development, oligodendrocyte precu
rsors originate in specific locations and subsequently migrate to all
regions of the CNS. Here, we demonstrate that the chick optic nerve is
populated by oligodendrocyte precursors, which initially appeared in
a focal region at the ventral midline of the third ventricle at stage
26-27. Oligodendrocyte precursors migrated into the chiasmal end of th
e nerve by stage 29 and became uniformly distributed by stage 35. Migr
ating precursors were restricted to the anterior region of the nerve,
closely apposed to axons, and had a bipolar morphology. In contrast to
the polysialic acid (PSA)-dependent cooperative streaming migration o
f olfactory neuronal precursors, the migration of oligodendrocyte prec
ursors along the optic nerve appeared axophilic and unaffected by remo
val of neural cell adhesion molecule (N-CAM)-associated PSA. These dat
a indicate that during development, defined domains of the ventricular
zone give rise to distinct cell types that utilize discrete mechanism
s to navigate specific migrational pathways.