Slimmer roosting sites were studied at four maternity colonies of Indi
ana bats (Myotis sodalis) in northern Missouri. Colonies of Indiana ba
ts used two types of roosts, primary and alternate, that differed in i
ntensity of use, number, and probable function. Primary roosts were de
fined as roosts where use by >30 bats on more than one occasion was ob
served. The number of primary roosts per colony ranged from one to thr
ee. All primary roosts were in standing dead trees situated in trees e
xposed to direct sunlight. Alternate roosts were used by smaller numbe
rs of bats. These roosts included both living and dead trees that typi
cally were located within the shaded forest interior. Differences in p
atterns of use between types of roosts seemed to be influenced by weat
her conditions in that use of alternate roost trees increased during p
eriods of elevated temperature and precipitation. Indiana bats have sp
ecific requirements for roost sites, but also must be able to relocate
when loss of bark, tree fall, or other events render their current ro
ost sites unusable. Practices of forest management within the summer r
ange of Indiana bats should favor retention of large-diameter, mature,
and senescent trees.