BIOCONVERSION OF 5-CYANOPYRAZINE TO 5-HYDROXYPYRAZINE-2-CARBOXYLIC ACID WITH AGROBACTERIUM SP DSM-6336

Authors
WIESER M HEINZMANN K KIENER A
Citation
M. Wieser et al., BIOCONVERSION OF 5-CYANOPYRAZINE TO 5-HYDROXYPYRAZINE-2-CARBOXYLIC ACID WITH AGROBACTERIUM SP DSM-6336, Applied microbiology and biotechnology, 48(2), 1997, pp. 174-176
Citations number
15
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Applied microbiology and biotechnology → ACNP
ISSN journal
01757598
Volume
48
Issue
2
Year of publication
1997
Pages
174 - 176
Database
ISI
SICI code
0175-7598(1997)48:2<174:BO5T5A>2.0.ZU;2-X
Abstract
5-Hydroxypyrazine-2-carboxylic acid, a versatile building block for th e synthesis of new antituberculous agents, was prepared by whole-cell biotransformation from 2-cyanopyrazine via pyrazinecarboxylic acid usi ng Agrobacterium sp. DSM 6336. By developing a fermentation process fo r this two-enzyme-step bioconversion, a product concentration of 286 m M (40 g/l) was obtained. After the isolation method had been optimized the total yield was 80%.