ONE-STEP TRANSFORMATION OF THE DIMORPHIC YEAST YARROWIA-LIPOLYTICA

Authors
CHEN DC BECKERICH JM GAILLARDIN C
Citation
Dc. Chen et al., ONE-STEP TRANSFORMATION OF THE DIMORPHIC YEAST YARROWIA-LIPOLYTICA, Applied microbiology and biotechnology, 48(2), 1997, pp. 232-235
Citations number
20
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Applied microbiology and biotechnology → ACNP
ISSN journal
01757598
Volume
48
Issue
2
Year of publication
1997
Pages
232 - 235
Database
ISI
SICI code
0175-7598(1997)48:2<232:OTOTDY>2.0.ZU;2-U
Abstract
An efficient one-step transformation method for the dimorphic yeast Ya rrowia lipolytica is described. Using cells grown overnight on agar pl ates, the whole process is carried out within 1 h. The transformant cl ones could be recovered on selective plates as early as 36-48 h after plating, The efficiency was better than 10(5) transformants/mu g repli cative plasmid DNA. Effects of cell density, dithiothreitol, heat shoc k, poly(ethylene glycol) 4000 concentration and the wetness of selecti ve plates were investigated.