H. Semba et K. Sakano, A PARA-SITE-SPECIFIC HYDROXYLATION OF AROMATIC-COMPOUNDS BY MYCOBACTERIUM SP STRAIN-12523 - STABILIZATION OF THE HYDROXYLATION ACTIVITY, Applied microbiology and biotechnology, 48(2), 1997, pp. 256-260
Mycobacterium sp. strain 12523 has a parasite-specific hydroxylation a
ctivity, which produce para-substituted phenols from various aromatic
compounds. However, the activity is unstable and the reactions are ina
ctivated within 24 h. In order to extend the reaction period, the fact
ors that affected reaction stability were examined. The hydroxylation
activity of the cells incubated in buffer was significantly stabilized
by the inclusion of an inducer such as methyl ethyl ketone. It is sug
gested that a regulatory mechanism is involved in controlling the acti
vity. This study resulted in the development of a convenient method to
stabilize the hydroxylation activity, involving the addition of an in
ducer, such as acetone, to the reaction system. This method permitted
the hydroxylation reaction to continue for more than 67 h.