LC-BIOSENSOR SYSTEM FOR THE DETERMINATION OF THE NEUROTOXIN BETA-N-OXALYL-L-ALPHA,BETA-DIAMINOPROPIONIC ACID

An analysis system is described for the determination of the neurotoxi n beta-N-oxalyl-alpha,beta-diaminopropionic acid (beta-ODAP). The syst em is based on liquid chromatographic separation of beta-ODAP from int erfering amino acids on an anion exchange column coupled with an amper ometric enzyme electrode for the registration of beta-ODAP. The electr ode is based on a graphite rod modified with an Os2+/3+ redox polymer cross-linked with L-glutamate oxidase and horseradish peroxidase. This LC-bienzyme electrode analytical system enabled monitoring of as low as 4 mu M beta-ODAP (injection volume 100 mu L). The beta-ODAP content in real grass pea samples was measured to range between 3.3 and 5.2 g kg(-1) in dry grass pea.