DETECTION OF LACCASE, PEROXIDASE, AND POLYPHENOL OXIDASE ON A SINGLE POLYACRYLAMIDE-GEL ELECTROPHORESIS

Laccase, polyphenol oxidase and peroxidase are widely distributed in b oth plants and micro-organisms. Among these enzymes, the identificatio n of one particular activity in the presence of the others is often di fficult as enzymes can often oxidise the same substrates. A method is described, that is suitable to differentiate the three activities on t he same polyacrylamide gel electrophoresis. The method is based on the use of two substrates, 4-amino-N,N-diethylaniline and 4-tert-butyl-ca techol, so that coloured spots appear corresponding to the different a ctivities. Moreover, a comparison among different mushroom species bel onging to the same genus is reported using the described method.