RESOLUTION OPTIMIZATION IN ION-INTERACTION RP-HPLC

Since in ion-interaction chromatography the dependence of the retentio n on the concentration of the ion-interaction reagent (IIR) follows di fferent behaviours for the different analytes, the adjustment of IIR c oncentration can usefully be used to optimize resolution. As an exampl e, resolution between nitrate and p-aminophenol was investigated as a function of IIR concentration and mobile phase now-rate. The IIRs used are an aqueous solution of octylamine, brought to pH=6.4 with o-phosp horic acid.