EFFECTS OF EXTRACELLULAR ENVIRONMENT ON THE OSMOTIC SIGNAL-TRANSDUCTION INVOLVED IN ACTIVATION OF MOTILITY OF CARP SPERMATOZOA

The mechanism by which a hypo-osmotic shock activates motility of carp spermatozoa was studied. The direct role of osmolality at the axoneme was investigated after demembranation of spermatozoa with Triton X-10 0 and reactivation in various ionic or anionic solutions containing Mg -ATP: demembranated spermatozoa remain motile in solutions of osmolali ty up to 550 mOsm kg(-1) while non-demembranated spermatozoa are immot ile when osmolality rises above 250 mOsm kg(-1) with the same salt sol utions as well as in non-ionic solutions. Suspension in hypo-osmotic s aline solutions triggered the swelling of native carp spermatozoa. No motility or swelling occurred above 200-300 mOsm kg(-1) and this osmol ality is probably that of the cytosol. The swelling of carp spermatozo a is the result of an entrance of water but this was not affected by p CMBS, an inhibitor of the aquaporin CHIP28, or by various inhibitors o f the co-transport of water with ions. Various pharmacological agents that affect the motility of different sperm species had no effect on c arp sperm motility when used under similar conditions. However, prolon ged exposure to a solution devoid of K+ or Cl- affects the activation of motility in a reversible manner, suggesting that these ions have a role in the perception or transduction of the osmotic signal. Altering the concentration of intracellular second messengers such as Ca2+ and cAMP, and the FH did not affect the motility of carp spermatozoa. How ever, DMSO at 1-20% (400-3200 mOsm kg(-1)) affects the motility of car p spermatozoa 3-4 min after mixing. These results show that the activa tion signal of carp sperm motility differs from that known for spermat ozoa of other species of fish such as trout. Our results indicate that the activation mechanism may involve a co-transport of ions or specif ic 'stretch-activated channels' that are sensitive to osmotic pressure .