IN-VITRO STUDY OF THE EFFECT OF SODIUM-HYPOCHLORITE AND GLUTARALDEHYDE ON SUBSTRATE ADHERENCE CAPACITY OF MACROPHAGES

The purpose of this study was to investigate the in vitro effect of tw o irrigation solutions used in endodontics (5.25% sodium hypochlorite and 1% glutaraldehyde) on substrate adherence capacity of macrophages to determine if these substances can alter macrophage function. Inflam matory macrophages were obtained from Wistar rats and resuspended in R PMI-1640 medium. Substrate adherence capacity assays were carried out in Eppendorf tubes for 15 min of incubation at 37 degrees C in a humid ified atmosphere of 5% CO2. The adherence index was calculated. Result s showed that both sodium hypochlorite and glutaraldehyde significantl y decreased the substrate adherence capacity of inflammatory macrophag es. To take into account that adhesion is the first step in the phagoc ytic process of macrophages and in antigen presentation, sodium hypoch lorite and glutaraldehyde could inhibit macrophage function and reduce inflammatory reactions in periapical tissues when they are used in ro ot-canal therapy.