ENZYMES CATALYZED ESTERIFICATION ON N-PROTECTED AMINO-ACIDS WITH SECONDARY ALCOHOLS

Secondary alcohols were converted to their corresponding esters with N -Cbz-L-amino acids by Celite-immobilized protease or lipase in organic solvents at pH 7.5. The esterification of 2-butanol and 2-phenylethan ol were achieved up to 71% yields. The optimal reaction condition for Aspergillus oryzae protease (AOP) catalyzed synthesis of N-Cbz-L-aspar tyl-sec-butyl ester (97.6 de) was at pH 7.5 and a duration of 96 hours .