REVERSE HYDROLYSIS CATALYZED BY BETA-N-ACETYLHEXOSAMINIDASE FROM ASPERGILLUS-ORYZAE

Authors
RAJNOCHOVA E DVORAKOVA J HUNKOVA Z KREN V
Citation
E. Rajnochova et al., REVERSE HYDROLYSIS CATALYZED BY BETA-N-ACETYLHEXOSAMINIDASE FROM ASPERGILLUS-ORYZAE, Biotechnology letters, 19(9), 1997, pp. 869-872
Citations number
14
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
19
Issue
9
Year of publication
1997
Pages
869 - 872
Database
ISI
SICI code
0141-5492(1997)19:9<869:RHCBBF>2.0.ZU;2-A
Abstract
beta-N-Acetylhexosaminidase from Aspergillus oryzae catalyzes the reve rse glycosylation of N-acetylglucosamine (1.5 M) to GlcNAcp beta 1-->6 GlcNAcp in 14% yield. Addition of salts [LiCl, (NH4)(2)SO4 0.1-1 M] lo wers the water activity in the system thus increasing the yield of the thermodynamically controlled reaction. Benzylalcohol as acceptor give s under the same conditions benzyl O-beta-D-2-acetamido-2-deoxy-glucop yranoside.