OPTIMIZATION OF THE REACTION MEDIUM FOR ESTERIFICATION CATALYZED BY A-LIPASE FROM PSEUDOMONAS-FLUORESCENS

Authors
TWEDDELL RJ KERMASHA S COMBES D MARTY A
Citation
Rj. Tweddell et al., OPTIMIZATION OF THE REACTION MEDIUM FOR ESTERIFICATION CATALYZED BY A-LIPASE FROM PSEUDOMONAS-FLUORESCENS, Biotechnology letters, 19(9), 1997, pp. 939-942
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
19
Issue
9
Year of publication
1997
Pages
939 - 942
Database
ISI
SICI code
0141-5492(1997)19:9<939:OOTRMF>2.0.ZU;2-Z
Abstract
The performance of a crude extract lipase from Pseudomonas fluorescens in esterification was evaluated in microaqueous, biphasic and surfact ant-enriched biphasic systems containing various amounts of water (fro m almost no water to pure water). The results showed a strong negative influence of the water content on the thermodynamic equilibrium of th e reaction in biphasic systems. From a kinetic point of view, the enzy me was more efficient in systems involving a water/organic solvent int erface (4 times in the biphasic system, 12 times in the surfactant-enr iched biphasic system).