AN OPTIMIZED METHOD FOR THE QUANTITATION OF CARBOXYAMIDO-TRIAZOLE (CAI) IN HUMAN PLASMA WITH SOLID-PHASE EXTRACTION AND REVERSED-PHASE HPLC

The anticancer agent CAI was quantitated from human plasma using a C-1 8 column with a mobile phase consisting of acetonitrile and water (eac h containing 0.01 M ammonium acetate) pumped over a gradient at a now rate of 1.0 mL/min. The detection of CAI and the internal standard, ha rmine, was accomplished using a photodiode-array detector set at 264 a nd 323 nm, respectively. The chromatographic run time was 17.5 minutes . The plasma samples were prepared for HPLC analysis using a C-18 soli d phase extraction procedure. Two calibration curves were prepared, an d the assay was shown to be linear in the concentration range of 0.02 to 0.5 mu g/mL for the low curve and 0.25 to 10.0 mu g/mL for the high curve, with average correlation coefficients of 0.9933 and 0.9938, re spectively. Intraassay and Inter-assay imprecisions were less than 10. 0% with an error of accuracy of less than 11.0%. The assay was reprodu cible with the sensitivity needed for the prediction of CAI levels in the plasma of cancer patients.