The development of new approaches to improve breeding efficiency of tr
opical forage grasses is of great importance. The most recently develo
ped techniques to study the type of apomixis and to establish the mode
s of reproduction in progeny of apomictic plants i.e.: callose deposit
ion test, clearling methods, intact embryo sac analysis, flow cytometr
y, molecular approaches and the methods of classiacal light and transm
ission electron microscopy are described and discussed. Cytological an
d functional aspects of apospory with following aposporous parthenogen
esis, and diplospory in grasses are highlighted.